Laser Scanner
Amersham Typhoon RGB Biomolecular Imager, Cytiva
The Typhoon RGB is an extremely sensitive laser scanner that detects, documents, and quantifies radiolabeled and fluorescently labeled proteins and nucleic acids. For phosphorimaging, a phosphor storage screen is exposed to a radioactive 2D sample, such as a gel or blot, capturing the energy from the emitted radiation. The phosphor screen is then imaged by the scanner, which exposes the screen to additional light, releasing the captured energy as light in proportion to the amount of radioactivity in the sample.
Features
- Compatible with most radioisotopes including 3H, 11C, 14C, 18F, 32P, 33P, 35S, 99mTc, 125I
- Excitation at 488, 532 and 635 nm
- Extremely sensitive: able to detect as little as 3 pg of protein
- A dynamic range of more than 5 orders of magnitude
- Higher sensitivity and reduced exposure time compared to traditional film
- Pixel resolution down to 10 μM
- Maximum field of view: 40 x 46 cm
- Post capture image processing (crop, rotation, contrast, and brightness adjustment) to enhance images for qualitative purposes
- Software to analyze and quantify images, includes automatic lane determination and band detection, background subtraction
Applications
- Image and quantify radiolabeled proteins and nucleic acids on blots or gels
- Fluorescent imaging of gels and blots: Western, Northern, Southern blotting, etc.
- High resolution imaging of other radioactive or fluorescent 2D samples, such as tissue sections
- Densitometry for quantification of colorimetrically stained gels or blots
Internal Rates
- Unassisted: $25.00/hr
- Assisted: $88.00/hr