Protein Purification System
AKTA Pure 25 M1 Protein Purification System, GE Healthcare
AKTA pure is a versatile automated liquid chromatography system for the fast purification of proteins, peptides, nucleic acids, and other biomolecules to microgram to gram levels. The system supports a broad range of chromatography techniques, allowing separation based on size, shape, charge, hydrophobicity, and non-covalent interaction. A selection of columns and sample loops are available to AKTA users.
Features
- Capable of flow rates from 0.001 to 25 mL/min and a max system pressure of 20 MPa
- Simultaneously monitor three wavelengths between 190 and 700 nm
- Pre-column and post-column pressure sensors to protect column hardware and resin
- Pre-column air sensor to protect columns
- Column valve to allow simultaneous attachment of up to five columns
- Flow direction reversal to aid in column cleaning
- Four inlet lines allow use of four different solutions without manual intervention
- Equipped with fraction collector, which can fractionate by time, volume, or automatic peak recognition
- Latest version of Unicorn software, which features improved, intuitive method building and data analysis
- System redesigned with forward facing wet system to allow easier access
- Compatible with all GE columns and resins as well as other commercial columns
Columns & Sample Loops Available
- Size exclusion:
- Superdex 200 Increase 10/300 GL: high resolution, small scale purification of 10 to 600 kDa proteins, analysis of oligomeric state of antibodies and other proteins
- HiPrep Sephacryl S-200 HR: preparative purification of 5 to 250kDa proteins
- Ion exchange:
- SP Sepharose HP: high resolution strong cation exchange
- Q Sepharose HP: high resolution strong anion exchange
- Affinity:
- HisTrap HP: nickel-charged IMAC for high resolution, his-tagged protein purification
- 500 µL and 2 mL capillary loops: reproducible, small volume application of sample
- 50 mL superloop: large volume sample application with minimal sample loss, allows repeated injections without manual intervention
Applications
- Purification of biomolecules, such as proteins, peptides, and nucleic acids, for subsequent detailed studies of structure, function, and/or binding to other biomolecules
- Structurally characterize the size, shape, and/or charge properties of a novel biomolecule relative to known standards
Internal Rates
- Unassisted: $17.70/hr
- Assisted: $68.70/hr