Real-Time Polymerase Chain Reaction System
QuantStudio 3 RT-qPCR System, Thermo Fisher Scientific
Real-time polymerase chain reaction is a sensitive technique for the detection and quantification of nucleic acids. In real-time qPCR, a DNA target is amplified by PCR and the amount of product is monitored during the reaction by measuring the fluorescence of dyes or probes. Fluorescent dyes that bind dsDNA or sequence-specific DNA probes labeled with a fluorescent reporter are used for detection. Real-time qPCR can be used to quantify the absolute amount of starting material or to compare the relative amounts of a target gene between different samples.
Features
- 96-well 0.1 mL format
- 4 filters for detection. Excitation range: 450-600 nm; emission range: 500-640 nm
- Compatible dyes: FAM, SYBR Green, VIC, JOE, HEX, TET, ABY, NED, TAMRA, Cy3, JUN, ROX, Texas Red
- Multiplexing of up to four targets
- 3 VeriFlex temperature control zones
- Max block ramp rate of 9°C/sec
Applications
- Monitor gene expression
- SNP genotyping
- Pathogen detection
- Quantify markers for cancer and genetic diseases
- Protein thermal shift assays
- Melt curve analysis for PCR product quality control
Internal Rates
- Unassisted: $18.80/hr
- Assisted: $69.80/hr