Preparative Ultracentrifuge

Optima XPN-90 Preparative Ultracentrifuge, Beckman Coulter

XPN-90 300

Floor preparative ultracentrifuge that separates components of differing densities using high centripetal forces.

Features

  • Temperature control: 0°C to 40°C in 1°C increments
  • Instrument max speed of 70,000 RPM and max capacity of 560 mL

Applications

  • Separation of proteins by ammonium sulfate precipitation and sucrose/glycerol gradients
  • Clarification of lysates and homogenates
  • Fractionation of lipoproteins by differential floatation and density gradients
  • Isolation of subcellular fractions (microsomes, mitochondria, nuclei, membranes, ribosomes) by pelleting
  • Isolation of subcellular fractions (chromatin, mitochondria, ribosomes, microsomes, membranes) by sucrose gradients
  • DNA size fractionation in gradients and plasmid purification in CsCl
  • Pelleting in CsCl and CsTFA density gradients of RNA
  • Isolation of viruses by pelleting and density gradients

Rotors Available

Type 70 Ti

  • Max speed: 70,000 RPM
  • Max capacity: 312 mL (8 x 39 mL)
  • Applications: Rapid pelleting of subcellular fractions, viruses, viral particles, RNA; separation of lipoproteins, plasmid DNA

Type 45 Ti

  • Max speed: 45,000 RPM
  • Max capacity: 564 mL (6 x 94 mL)
  • Applications: Separation of large volumes of subcellular fractions and viruses

SW 60 Ti

  • Max speed: 60,000 RPM
  • Max capacity: 24 mL (6 x 4 mL)
  • Applications: Rate-zonal separation of subcellular fractions, proteins, RNA, viruses; rapid pelleting of particles

SW 32 Ti

  • Max speed: 32,000 RPM
  • Max capacity: 231 mL (6 x 38.5 mL)
  • Applications: Separation of viruses and subcellular particles in density gradients

SW 32.1 Ti

  • Max speed: 32,000 RPM
  • Max capacity: 102 mL (6 x 17 mL)
  • Applications: Separation of viruses and subcellular particles in density gradients

Internal Rates

  • Unassisted: $10.50/hr or $56/day
  • Assisted: $53.50/hr or $99/day

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