Preparative Ultracentrifuge
Optima XPN-90 Preparative Ultracentrifuge, Beckman Coulter
Floor preparative ultracentrifuge that separates components of differing densities using high centripetal forces.
Features
- Temperature control: 0°C to 40°C in 1°C increments
- Instrument max speed of 70,000 RPM and max capacity of 560 mL
Applications
- Separation of proteins by ammonium sulfate precipitation and sucrose/glycerol gradients
- Clarification of lysates and homogenates
- Fractionation of lipoproteins by differential floatation and density gradients
- Isolation of subcellular fractions (microsomes, mitochondria, nuclei, membranes, ribosomes) by pelleting
- Isolation of subcellular fractions (chromatin, mitochondria, ribosomes, microsomes, membranes) by sucrose gradients
- DNA size fractionation in gradients and plasmid purification in CsCl
- Pelleting in CsCl and CsTFA density gradients of RNA
- Isolation of viruses by pelleting and density gradients
Rotors Available
Type 70 Ti
- Max speed: 70,000 RPM
- Max capacity: 312 mL (8 x 39 mL)
- Applications: Rapid pelleting of subcellular fractions, viruses, viral particles, RNA; separation of lipoproteins, plasmid DNA
Type 45 Ti
- Max speed: 45,000 RPM
- Max capacity: 564 mL (6 x 94 mL)
- Applications: Separation of large volumes of subcellular fractions and viruses
SW 60 Ti
- Max speed: 60,000 RPM
- Max capacity: 24 mL (6 x 4 mL)
- Applications: Rate-zonal separation of subcellular fractions, proteins, RNA, viruses; rapid pelleting of particles
SW 32 Ti
- Max speed: 32,000 RPM
- Max capacity: 231 mL (6 x 38.5 mL)
- Applications: Separation of viruses and subcellular particles in density gradients
SW 32.1 Ti
- Max speed: 32,000 RPM
- Max capacity: 102 mL (6 x 17 mL)
- Applications: Separation of viruses and subcellular particles in density gradients
Internal Rates
- Unassisted: $12.50/hr or $73.00/day
- Assisted: $63.50/hr or $124.00/day