Surface Plasmon Resonance System

Biacore T200 SPR, Cytiva

Biacore T200

Surface Plasmon Resonance (SPR) is a sensitive, label-free technique that monitors molecular interactions in real-time. In SPR, one interacting partner is immobilized onto a sensor surface and the other is flowed over the surface. Binding between the interaction partners leads to changes in mass near the sensor surface, which is detected by the instrument as subtle changes in refractive index.

SPR can be used to rapidly provide quantitative information about interactions between proteins, nucleic acids, lipids, drugs, inhibitors and other organic compounds, such as binding affinity, kinetics, specificity, and concentration. Thermodynamic parameters can also be quantified by SPR via temperature variation. SPR can analyze interactions in various solutions, from clear buffers to cell supernatants and blood plasma. 

Features

  • Temperature control (4 - 45°C) of sample compartment and during analysis
  • Compatible with 96- and 384-well plates
  • Ability to recover samples for subsequent identification by mass spectrometry
  • Four flow cells enabling unattended analysis of three immobilized biomolecules and a reference surface
  • Detection of wide size range of biomolecules from 100 Da to >1 MDa
  • Association rates: 1 x 103 -  3 x 109 M-1s-1
  • Dissociation rates: 1 x 10-5 - 1 s-1

Applications

  • Drug development
    • Compound screening, kinetic and affinity analysis of candidates, specificity evaluation, and lead optimization
  • Immunogenicity studies to detect anti-drug antibodies
  • Concentration measurements
  • Mapping binding sites on macromolecules
  • Basic research
    • Characterization of molecular interactions
    • Structural/functional studies

Publications

Publications resulting from use of the Biacore T200 SPR system should acknowledge NIH S10 shared instrumentation grant 1S10OD028553-01.

Internal Rates

  • Unassisted: $213/day
  • Assisted: Please contact

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